By George P. Studzinski
This significant textual content presents conceptual outlines and particular tactics for easy and complex stories of telephone loss of life through apoptis. Chapters at the reputation of apoptis as amazing from neurosis and nonspecific cellphone DNA harm are by way of a scientific exam of the proven and the central novel methodologies used by major laboratories accomplishing learn on apoptis. a large choice of methods are supplied, allowing readers to take part in state of the art research.
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Additional resources for Apoptosis: A Practical Approach (Practical Approach Series)
2 Problems in scoring apoptotic events Quantitation of apoptotic events in cellular/tissue systems is always a topic that raises much debate. e. cell deaths, and how to interpret such numbers (11,12). A number of factors can influence the frequency of apoptotic events, or 'apoptotic index'. These include: (a) Half-life. The half-life of apoptotic cells differs between cell systems/ tissues. In a tissue/cell line where apoptotic cells have a long half-life, samples at different times over a given period will show a higher frequency of events, compared with tissue where apoptotic cells have a short half-life, even though the absolute number of cell deaths may be the same.
16. , Sherman, Y. A. (1992). J. , 119,493. 38 2: Morphological recognition of apoptosis 17. A. (1993). J. PathoL, 170,1. 18. , Cornelisse, CJ. H. (1993). J. Histochem. , 41,7. 19. , Ohyama, H. and Yamada, T. (1997). Histochem. , 29,823. 20. , Nakayama, K. A. (1994). J. , 108,2261. 21. , Ansari, B. and Hopwood, D. (1994). J. , 107, 3569. 22. S. D. (1977). Ultrastruct. , 60, 272. 23. , Hvala, M. and Chun, J. (1998). , 5,702. 24. , Bultinck, J. and Herman, A. (1996). Atherosclerosis, 120,115 25. M.
Another precaution often overlooked is to establish precisely the diploid DNA content of the cells under investigation before determining the sub-Gl values, since tumour cell populations are not infrequently composed of a mixture of diploid and 15 George P. Studzinski Table 3. 92 NDb ND "Nuclear DNA and mtDNA levels were compared directly by sequential hybridization of the same membrane with probes for c-myc as a representative nuclear gene, and p72, a probe for mtDNA. Following densitometry of the autoradiographs, the ratios of the relative values of mtDNA/nuclear gene were obtained for untreated cells ('controls') and for each treated group.
Apoptosis: A Practical Approach (Practical Approach Series) by George P. Studzinski